X-RAY-MICROANALYSIS OF SINGLE AND CULTURED-CELLS

  • 1 January 1984
    • journal article
    • research article
    • p. 1875-1882
Abstract
X-ray microanalysis of single or cultured cells is often a useful alternative or complement to the analysis of the corresponding tissue. It also allows the analysis of individual cells in a cell population. Preparation for X-ray microanalysis poses a number of typical problems. Suspensions of single cells can be prepared by either of 2 pathways: washing-mounting-drying, or centrifugation-freezing or fixation-sectioning. The washing step in the preparation of single or cultured cells presents the most severe problems. Cultured cells are generally grown on a substrate that is compatible with both the analysis and the culture, washed and dried. In some cases, sectioning of cultured cell monolayers is performed. Special problems in quantitative analysis occur in those cases where the cells are analyzed on a thick substrate, since the substrate contributes to the spectral background.