Evaluation of Column Performance in Liquid Chromatography

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Abstract
Procedures for the evaluation of chromatographic properties of LC columns are described. General tests for assessing silanol activity, hydrophobicity, trace metal activity, phase type (monomeric vs. polymeric chemistry), and pore size are presented. Also included are specific tests for various solute classes. Emphasis is placed on practical aspects of column testing; characterization procedures that require bulk packing material (i.e., unpacking the column) have been largely excluded.

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