Applicability of Capillary Gas Chromatography to Systematic Toxicological Analysis: Occurrence of Concentration-Dependent Retention Behavior
- 1 July 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 7 (4) , 188-192
- https://doi.org/10.1093/jat/7.4.188
Abstract
The retention behavior for a selection of acidic, neutral, and basic drugs was examined on four fused silica capillary columns in a splitless injection mode. Mixtures of n-alkanes and dilsopropylamino-alkanes were also analyzed. The concentrations used ranged from 0.25–1000 ng/µL and injection volumes were either 2 or 4/µL. This corresponded to quantities of 1–4000 ng injected on the column. All substances showed concentration-dependent behavior: after being nearly constant or showing a decrease at low concentrations, retention times markedly increased when concentrations exceeded about 100 ng/µL (400 ng injected). At very high concentrations, peak splitting occurred. In the more pronounced cases, the differences caused by this concentration effect could reach values of over 100 retention-index units.This publication has 1 reference indexed in Scilit:
- Application of fused-silica capillary gas chromatography to the analysis of underivatized drugsJournal of Chromatography A, 1982