Acetylcholine receptors of theDrosophila brain: A 900 bp promoter fragment contains the essential information for specific expression of theard gene in vivo
- 13 June 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 346 (2-3) , 135-140
- https://doi.org/10.1016/0014-5793(94)00443-9
Abstract
Theard gene encodes a β‐subunit ofDrosophila nicotinic acetylcholine receptors specifically expressed in a subset of neurons. To identify thecis‐regulatory region responsible for this cell‐specific expression, various 5′ fragments of theard gene were fused to alacZ reporter gene and introduced into theDrosophila genome. A DNA fragment spanning ∼760 bp upstream and ∼140 bp downstream of a cluster of putative transcription start sites produced a pattern of β‐galactosidase activity that resembles the distribution of ARD transcripts. Both in embryos and adults the levels oflacZ RNA were similar to those of endogenous ARD transcripts, suggesting that the 900 bp fragment harbors all essential elements for proper expression of theard gene.Keywords
This publication has 42 references indexed in Scilit:
- Immunohistochemical localization of a ligand‐binding and a structural subunit of nicotinic acetylcholine receptors in the central nervous system of Drosophila melanogasterJournal of Comparative Neurology, 1993
- The Diversity of Neuronal Nicotinic Acetylcholine ReceptorsAnnual Review of Neuroscience, 1993
- Nicotinic acetylcholine receptors of the central nervous system of DrosophilaBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1992
- Cross-linking of 125I-α-bungarotoxin to Drosophila head membranes identifies a 42 kDa toxin binding polypeptideNeuroscience Letters, 1992
- How complex is the nicotinic receptor system of insects?Trends in Neurosciences, 1992
- Neuronal Nicotinic Acetylcholine Receptors in Drosophila: Antibodies Against an α‐Like and a Non‐α‐Subunit Recognize the Same High‐Affinity α‐Bungarotoxin Binding ComplexJournal of Neurochemistry, 1991
- Structure and developmental expression of the Dα2 gene encoding a novel nicotinic acetylcholine receptor protein of Drosophila melanogasterFEBS Letters, 1990
- Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.Proceedings of the National Academy of Sciences, 1988
- Characterization of an invertebrate nicotinic acetylcholine receptor gene: The ard gene of Drosophila melanogasterFEBS Letters, 1988
- Molecular properties and functions of insect acetylcholine receptorsJournal of Insect Physiology, 1987