Antibody responses to Brucella abortus 2308 in cattle vaccinated with B. abortus RB51
- 1 March 1996
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 64 (3) , 1030-4
- https://doi.org/10.1128/iai.64.3.1030-1034.1996
Abstract
Cattle vaccinated with Brucella abortus rough strain RB51 (SRB51) produced small amounts of serum immunoglobulin G (IgG) but no IgM antibody to smooth strain 2308 (S2308) bacteria and produced no IgG or IgM antibody to S2308 lipopolysaccharide (LPS). Western immunoblot analysis revealed that antiserum from SRB51-vaccinated cattle contained IgG antibody that reacted with S2308 proteins of 84 to <20 kDa. However, antiserum from the vaccinated cattle did not contain agglutinating B. abortus antibody in the tube agglutination test for brucellosis. These results suggest that SRB51-vaccinated cattle produced no antibody to S2308 LPS, although they did produce nonagglutinating IgG antibody that reacted with S2308 bacteria and bacterial proteins of 84 to <20 kDa.Keywords
This publication has 14 references indexed in Scilit:
- Comparative analysis of immune responses in cattle vaccinated with Brucella abortus strain 19 or strain RB51Veterinary Immunology and Immunopathology, 1995
- Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortusAmerican Journal of Veterinary Research, 1993
- Bacterial survival, lymph node changes, and immunologic responses of cattle vaccinated with standard and mutant strains of Brucella abortusAmerican Journal of Veterinary Research, 1992
- Experimental infection of goat fetuses in utero with a stable, rough mutant of Brucella abortusResearch in Veterinary Science, 1991
- Biological properties of RB51; a stable rough strain of Brucella abortusVeterinary Microbiology, 1991
- Immunoglobulin G binding activity of Brucella abortusMolecular Immunology, 1991
- Effect of bovine non-agglutinating antibodies on the blood clearance of 131I-labelled Brucella abortus strain 45/20Veterinary Microbiology, 1987
- Detection of antigenic fractions from Brucella abortus S45/20 which bind to non-agglutinating antibodies using electroblotting and enzyme-linked antibody probesVeterinary Microbiology, 1986
- Analysis and in vivo assay of B. abortus agglutinating and non-agglutinating antibodiesVeterinary Microbiology, 1984
- IMMUNOGLOBULIN CLASS SPECIFICITY OF NON‐AGGLUTINATING ANTIBODY PRODUCED IN CATTLE FOLLOWING BRUCELLA ABORTUS 45/20 VACCINATIONAustralian Veterinary Journal, 1975