Chromosome Analysis of a Spontaneous, Transplantable Golden Hamster Tumor

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Abstract
The chromosome constitution of nine “H 10” golden hamster sarcomas was analyzed. The distribution of chromosome counts in 845 metaphases revealed a diploid stemline. This represented an average of 66 percent of the dividing cell population. With the use of the easily recognizable X chromosome as a cytological marker, it was determined that the diploid and near-diploid cells originated in the host and did not grow in a homologous animal. The analysis of 84 karyotypes showed the existence of pseudodiploidy and chromosomal changes in some of the diploid and near-diploid cells. The polyploid cells had a mode of 85–102, predominantly 96 chromosomes, and were characterized by a high number of X chromosomes. These cells with similar chromosome constitution were considered to be serially transferred from animal to animal, and it was thought that the large number of X chromosomes might give a selective advantage for transplantation. The high proportion of diploid and near-diploid cells in all tumors examined is discussed and viral induction of growth in the host animal is suggested.

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