Evaluation of Detergent Enzymes in Air

Abstract
To assist in evaluation of the physiological aspects of exposure to detergent enzymes and the adequacy of industrial hygiene controls, a method for determining air concentrations of proteolytic enzymes was developed. Samples are taken by means of a high-volume sampling technique in which the dust is collected on glass fiber pads. The total dust level is determined gravimetrically; the amount of proteolytic enzyme is determined colorimetrically with dimethylated casein as the substrate and trinitrobenzene sulfonic acid sis the color reagent. Data describing analytical accuracy, precision, and detection limits are presented. Results over a 16-month period for key operating areas indicate a reduction in total dust and enzyme levels as well as a satisfactory position in terms of the TLV. An automated data handling system which serves individual plant and corporate needs is described.

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