Pyruvate Kinase Isozymes among Human Organs and Blood Cells

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Abstract
In extracts of human tissues 3 different pyruvate kinases were demonstrated by acetate gel electrophoresis. Treatment with reduced glutathione resulted in an alteration of electrophoretic mobility of extracts from kidney and testis. The existence of interconvertible forms of M2 pyruvate kinase (PK) was suggested. Lymphocytes, polymorphonuclear cells and platelets were M2 type PK. An electrophoretic slow form was demonstrated in myeloblasts and lymphoblasts.

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