Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing
- 1 October 1993
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 365 (6447) , 671-673
- https://doi.org/10.1038/365671a0
Abstract
A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.Keywords
This publication has 6 references indexed in Scilit:
- DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragmentsNucleic Acids Research, 1992
- Human immunodeficiency virus type 1 mutants resistant to nonnucleoside inhibitors of reverse transcriptase arise in tissue culture.Proceedings of the National Academy of Sciences, 1991
- Zidovudine resistance predicted by direct detection of mutations in DNA from HIV-infected lymphocytesAIDS, 1991
- Multiple Mutations in HIV-1 Reverse Transcriptase Confer High-Level Resistance to Zidovudine (AZT)Science, 1989
- An lmproved method for directly sequencing PCR-amplified material using dimethyl sulphoxideNucleic Acids Research, 1989
- Fluorescence detection in automated DNA sequence analysisNature, 1986