Isolation of Intracellular Parasites (Plasmodium falciparum) from Culture Using Free-Flow Electrophoresis: Separation of the Free Parasites According to Stages
- 1 June 1982
- journal article
- research article
- Published by JSTOR in Journal of Parasitology
- Vol. 68 (3) , 443-450
- https://doi.org/10.2307/3280956
Abstract
Parasitized human erythrocytes were concentrated from continuous cultures of P. falciparum from 5-7% up to 80-95% using Plasmagel. After aggregation of the cells with phytohemagglutinin, the aggregated erythrocytes were fragmented by passing them, with minimal force, through successive nylon filters of decreasing pore size (100-3 .mu.m). The mixture of liberated, free parasites, intact erythrocytes and erythrocyte membrane vesicles was separated using free-flow electrophoresis. Most of the fractions containing free parasites did not show contamination with erythrocyte constituents as determined by light microscopy and EM, polyacrylamide gel electrophoresis and enzymatic analysis. The various stages of free parasites of P. falciparum exhibited different electrical surface charges. Rings and trophozoites were highly negatively charged whereas schizonts and, in particular, merozoites showed low negative charges. The various stages could be isolated separate from each other.This publication has 11 references indexed in Scilit:
- Stage-specific proteins and glycoproteins of plasmodium falciparum: identification of antigens unique to schizonts and merozoites.Proceedings of the National Academy of Sciences, 1980
- Factors affecting the ability of isolated Plasmodium knowlesi merozoites to attach to and invade erythrocytesParasitology, 1980
- Antigenicity of the infected-erythrocyte and merozoite surfaces in Falciparum malaria.The Journal of Experimental Medicine, 1979
- Characterization of a protein correlated with the production of knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparumProceedings of the National Academy of Sciences, 1979
- Free-flow electrophoresis for the separation of malaria infected and uninfected mouse erythrocytes and for the isolation of free parasites (Plasmodium vinckei): A new rapid technique for the liberation of malaria parasites from their host cellsZeitschrift Fur Parasitenkunde-Parasitology Research, 1979
- Concentration from Continuous Culture of Erythrocytes Infected with Trophozoites and Schizonts of Plasmodium Falciparum *The American Journal of Tropical Medicine and Hygiene, 1978
- Separation of viable schizont-infected red cells ofPlasmodium falciparumfrom human bloodPathogens and Global Health, 1978
- Surface properties of extracellular malaria parasites: electrophoretic and lectin-binding characteristicsInfection and Immunity, 1976
- Human Malaria Parasites in Continuous CultureScience, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976