A 13C double-filtered NOESY with strongly reduced artefacts and improved sensitivity
- 1 June 1995
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 5 (4)
- https://doi.org/10.1007/bf00182287
Abstract
A 1H NOESY experiment with two 13C half-filters is described which has, compared to previously reported versions, an enhanced overall sensitivity and strongly reduced intramolecular cross peaks in any part of the spectrum edited for intermolecular NOEs. By adding a shaped 13C pulse to the half-filter which selectively inverts the aromatic resonances, the filter can be tuned separately and simultaneously for the aliphatic and aromatic regions. Contrary to recently proposed schemes, no magnetization is destroyed, so that full sensitivity is retained for symmetric systems such as homodimers. Furthermore, by replacing the rectangular 180° 13C pulses by high-power hyperbolic secant pulses for inversion of the complete 13C spectral range, offset effects (which are another source of signal loss and artefacts) are eliminated. The spectra edited for intermolecular NOEs clearly demonstrate that residual artefacts are considerably smaller than in the original version of the experiment.Keywords
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