Determination of Aflatoxins in Peanut Products Using Reverse Phase HPLC
- 1 August 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 16 (8) , 372-376
- https://doi.org/10.1093/chromsci/16.8.372
Abstract
A high performance liquid chromatographic (HPLC) method is described for the determination of the four major aflatoxins, B1, B2, G1, and G2, in peanut products. The aflatoxins are extracted by adapting a procedure developed by Pons (1) at the SRRC, USDA, and quantitated utilizing a new 5 μm reverse-phase column with NaCl/acetonitrile/methanol mobile phase (3 +1 + 1). The 5 μm column achieved baseline resolution of each of the four aflatoxins. Retention times and peak heights were reproducible. The procedure was successfully applied to several types of peanut products and was applicable to both roasted and unroasted peanuts, which is a decided advantage over the current CB and BF extraction methods. Additionally, it can be used for sweetened peanut matrixes with no interferences in the chromatography. The total time required for sample preparation and aflatoxin determination is less than 1.5 hours.This publication has 1 reference indexed in Scilit:
- High Pressure Liquid Chromatographic Determination of Aflatoxins in Wines and Other Liquid ProductsJournal of AOAC INTERNATIONAL, 1977