Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays
Open Access
- 1 June 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (6) , 3169-3171
- https://doi.org/10.1128/aem.68.6.3169-3171.2002
Abstract
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.Keywords
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