Stimulation of messenger Ribonucleic acid synthesis in isolated nuclei by a protein that stimulates RNA polymerase II

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Abstract
A factor stimulating RNA polymerase II purified from [mouse] Ehrlich ascites tumor cells stimulated .alpha.-amanitin-sensitive RNA synthesis in nuclei isolated from spleen cells of anemic mice, although less than it stimulated purified RNA polymerase II. The fidelity of the resulting RNA synthesis was monitored by measuring the stimulation of globin mRNA synthesis. Globin mRNA was measured quantitatively by DNA-RNA hybridization by using plasmid DNA containing globin DNA sequences. The synthesis of globin mRNA was enhanced in isolated nuclei in the presence of this factor, coinciding with an increase of overall .alpha.-amanitin-sensitive RNA synthesis. An externally added factor did not stimulate random transcription but did stimulate meaningful RNA synthesis in isolated nuclei.