Properties of α1-antitrypsin secreted by human adenocarcinoma cell lines
- 16 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 328 (3) , 291-295
- https://doi.org/10.1016/0014-5793(93)80946-r
Abstract
α1‐Antitrypsin; (α1‐AT) produced by various human carcinoma (non‐hepatoma) cell lines were analyzed. Five out of eight cell lines secreted detectable amounts of α1‐AT into the conditioned media. All were adenocarcinoma cell lines. The tumor cell‐derived α1‐ATs had higher molecular weights (MW) than the normal plasma form. Most of this difference was an overall reflection of altered N‐glycosylation. As judged by binding of lectins, the glycosylation had shifted towards higher levels of triantennary oligosaccharides and higher levels of fucosylation. The conditioned media also contained lower MW α1‐AT species, possibly, proteolytically cleaved forms.Keywords
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