Behavior of Chromatography Columns with a Varying Stationary Phase: I. Segmented Liquid Phase Loadings

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Abstract
The chromatographic performance of columns comprised of segments with different liquid phase loading is evaluated with respect to efficiencies, resolution, and solute capacity factors. Columns were constructed of segments having 10-5-3-1% and 15-5-3-2% Carbowax 20M coated on 80/100 mesh Chromosorb W and 10-5-3-1% SP2100 on 100/120 mesh Supelcoport. Their behavior as a function of flow direction was studied. Special advantages with respect to separability or analysis time can be selected by a choice of flow direction. Operation of a segmented loaded column in a high-to-low-percent loading configuration may provide increased sample capacity and improved separation whereas the reverse configuration, under the same experimental conditions, offers reduced retention and greater column efficiency.

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