METHOD OF MASS CULTIVATION OF TOXOPLASMA-GONDII IN CELL-CULTURE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 29  (4) , 432-434
Abstract
Large quantities of T. gondii were cultivated in human larynx carcinoma cells (H.Ep 2, heteroploid). Virulent T. gondii strain RH were obtained from the peritoneal exudate of mice 2 days after infection served as inoculum. As culture medium for the cells and for growth medium, Minimum Essential Medium (MEM) supplemented by 5% fetal calf serum and 200,000 Iu/1 Pencillin plus 20 mg/l Sulmycin was used. Two to three days after inoculation, 150-200 times the amount of the inoculated T. gondi could be harvested in one or several harvests.