DETECTION OF POLIOVIRUS ANTIGENS AND ANTIBODIES - MICRO-INDIRECT HEMAGGLUTINATION AND HEMAGGLUTINATION INHIBITION TESTS FOR POLIOVIRUS TYPE-1, TYPE-2 AND TYPE-3

  • 1 January 1976
    • journal article
    • research article
    • Vol. 16  (63) , 29-36
Abstract
Microtiter indirect hemagglutination (IHA) and IHA-inhbition (IHAI) procedures were adapted to determine the reactivities of type I, II and III poliovirus antibodies and antigens. Glutaraldehyde-fixed sheep erythrocytes were sensitized for these tests with concentrated, partially purified preparations of type I, II and III poliovirus. Antibody titers by IHA were generally 10-100 times greater than serum microneutralization (SN) titers. The SN and IHA reactivities of 3 kinds of sera were compared. Of these sera, virus type specific antibodies in monospecific guinea pig sera 1 wk after immunization and in sera from hyperimmunized horses could be readily differentiated and measured; antibodies in human diagnostic specimens showed some intertypic cross-reactivity. Monovalent 1 wk immune guinea pig sera reacted specifically in the IHAI test to differentiate viruses, and could be used for virus typing and differentiating strains of poliovirus type III.