DIRECT RADIOIODINATION OF ESTRADIOL FOR A SENSITIVE RADIOIMMUNOASSAY
- 1 October 1979
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 49 (4) , 655-657
- https://doi.org/10.1210/jcem-49-4-655
Abstract
17 β-estradiol (E2) was iodinated with the chloramine-T method. The 125I-labelled material was purified with thin-layer chromatography. The major fraction was applied to a radioimmunoassay with an antiserum against estradiol-6-(O-carboxymethyl)oxime-BSA. The sensitivity of the assay was comparable to assays with 3H-E2 or 125I-nistamine-E2 as tracers. The working antiserum dilution (1:1 000 000) was the same with 125i-E2 and 3H-E2 but lower than that with 125I-histamine-E2. The results indicate that iodination of the A-ring of estrogens will not cause significant reduction of the immunoreactivity when combined with an antiserum to a B-ring conjugate.Keywords
This publication has 2 references indexed in Scilit:
- The use of iodinated steroid as radioligand for testosterone radioimmunoassayThe Journal of Steroid Biochemistry and Molecular Biology, 1978
- The measurement of digitoxin in human serum by radioimmunoassayJournal of Clinical Investigation, 1968