Resistance against cucumber mosaic virus in plants expressing the viral replicon
- 22 January 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 379 (1) , 26-30
- https://doi.org/10.1016/0014-5793(95)01477-2
Abstract
CMV RNAs 1 and 2 are considered to constitute the viral replicon. Tobacco plants were transformed with either RNA1 or RNA2 to produce plant lines V1 and V2, respectively. Plants homozygous for each of the RNAs were generated and crossed to produce V1V2 (V2V1) lines that expressed both RNA1 and RNA2. An RNase protection assay indicated that RNA1 and RNA2 multiplied in V1V2 (V2V1) plants. Surprisingly, V1V2 (V2V1) plants, unlike their parent lines, showed a remarkably high level of resistance to CMV; this resistance was more effective against RNA inoculation than against virion inoculation. Experiments using protoplasts showed that the resistance was expressed at the single cell level. All the data together suggested that the observed resistance does not fit the criteria for either ‘RNA-mediated’ or ‘replicase-mediated’ resistanceKeywords
This publication has 18 references indexed in Scilit:
- Homology‐dependent resistance: transgenic virus resistance in plants related to homology‐dependent gene silencingThe Plant Journal, 1995
- Replication of Cowpea Mosaic Virus RNA1 or RNA2 Is Specifically Blocked in TransgenicNicotiana benthamianaPlants Expressing the Full-Length Replicase or Movement Protein GenesMolecular Plant-Microbe Interactions®, 1995
- Transgenic Potato Virus Y Resistance in Potato: Evidence for an RNA-Mediated Cellular ResponsePhytopathology®, 1995
- Nucleotide sequence and infectivity of cucumber mosaic cucumovirus (strain K) RNA2 involved in breakage of replicase-mediated resistance in tobaccoJournal of General Virology, 1994
- RNA-Mediated Virus Resistance in Transgenic Plants: Exploitation of a Cellular Pathway Possibly Involved in RNA DegradationMolecular Plant-Microbe Interactions®, 1994
- Expression of the gene encoding the coat protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several different CMV strainsGene, 1991
- Expression of cucumber mosaic virus satellite RNA from cDNA copies in transgenic tobacco plants.Japanese Journal of Phytopathology, 1989
- Inoculation with RNAs 1 and 2 of Cucumber Mosaic Virus Induces Viral RNA Replicase Activity in Tobacco Mesophyll ProtoplastsJournal of General Virology, 1988
- Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or its Antisense RNANature Biotechnology, 1988
- Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation methodFEBS Letters, 1987