Field inversion gel electrophoresis with different pulse time ramps
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (21) , 6299-6304
- https://doi.org/10.1093/nar/18.21.6299
Abstract
The influence of different pulse time ramps on the separation of yeast chromosomes with field inversion gel electrophoresis (FIGE) was investigated by the means of two dimensional gel electrophoresis. The problem of band inversion, which makes it difficult to distinguish DNA molecules of different size, has been solved by using double randomized pulse times. A major disadvantage of the field inversion technique is thereby overcome, making this system comparable to other pulsed field techniques.This publication has 36 references indexed in Scilit:
- DNA trapping electrophoresisNature, 1990
- Velocity of DNA in gels during field inversionPhysical Review A, 1989
- Transverse alternating field electrophoresis and applications to mammalian genome mappingElectrophoresis, 1989
- Effect of electric field switching on the electrophoretic mobility of single-stranded DNA molecules in polyacrylamide gelsElectrophoresis, 1989
- High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 1. DNA size standards and the effect of agarose and temperatureBiochemistry, 1988
- High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 2. Effect of pulse time and electric field strength and implications for models of the separation processBiochemistry, 1988
- BBC microcomputer controlled field inversion gel electrophoresisBioinformatics, 1988
- Direct detection of more than 50% of the Duchenne muscular dystrophy mutations by field inversion gelsNature, 1987
- Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric FieldScience, 1986
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984