Physical development of tissue-reacted cationic iron colloid with protein silver and gold chloride
Open Access
- 1 January 1997
- journal article
- Published by Okayama Medical Association in Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
- Vol. 109 (7) , 151-156
- https://doi.org/10.4044/joma1947.109.7-12_151
Abstract
Rat brain sections were treated with fine granular cationic iron colloid, which was prepared by boiling ferric chloride with hydrazine hydrate and cacodylic acid (Murakami et al., 1986). The sections were then treated with ferrocyanide and HCl for Prussian blue reaction or with protein silver and gold chloride for physical development. Physical development had an advantage over Prussian blue reaction in that it produces clear and highly contrasted images under a light microscope. Thus, the perineuronal sulfated proteoglycans and the fine processes of the nerve cells were clearly demonstrated by physical development.Keywords
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