IGG AND COMPLEMENT RECEPTORS ON PURIFIED MOUSE EOSINOPHILS AND NEUTROPHILS

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (4) , 779-786
Abstract
Mouse eosinophil and neutrophil receptors for IgG and complement (c) were examined by rosette formation, phagocytosis and 51Cr release assays, using mouse monoclonal antibodies and C-coated sheep erythrocytes. Mouse eosinophils and neutrophils form a high number of rosettes in the presence of mouse C; eosinophils show a higher requirement for C molecules. Both types of granulocyte phagocytose C-coated sheep erythrocytes very actively, although low levels of 51Cr release are obtained. Eosinophils and neutrophils show higher activity in the presence of IgG2b than in the presence of IgG1. While both cell types are similarly active when the former antibody is used, neutrophils are the more active when IgG1 is used. It remains uncertain whether this is a result of the higher binding obtained with IgG2b monoclonal antibody. Both cell types behave similary at high antibody concentrations but neutrophils are the more active at high antibody dilutions. The 51Cr release assay is superior to the rosette assay as it allows comparisons between eosinophils and neutrophils at high antibody concentrations. Highest values of phagocytosis of opsonized red cells are obtained after 5 min rather than the 1/2-1 h incubation periods normally used.

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