ADP-Ribosylation of Membrane Proteins in Cholinergic Nerve Terminals
- 1 April 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 38 (4) , 1080-1086
- https://doi.org/10.1111/j.1471-4159.1982.tb05351.x
Abstract
Lysed Torpedo synaptosomes or washed synaptosomal membranes were incubated with [32P]NAD+ and subjected to electrophoresis on sodium dodecyl sulfate polyacrylamide gels. More than 8 membrane proteins were ADP-ribosylated. The most intensely labeled proteins were those of MW = 62,000 and 82,000. Radiolabeling was more intense in synaptosomes than in other subcellular fractions. Cholera toxin caused ribosylation of additional synaptosomal proteins with MW = 42,000 and (in some preparations) 49,000. Neither endogenous nor cholera toxin-catalyzed ADP-ribosylation required added guanyl nucleotides. Cholera toxin increased the adenylate cyclase activity of synaptosomal membranes, suggesting that the cholera toxin substrates are regulatory components of adenylate cyclase in these synaptosomes.Keywords
This publication has 33 references indexed in Scilit:
- Endogenous and cholera toxin-catalyzed ADP-ribosylation of a plasma membrane protein of RL-PR-C cloned rat hepatocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1981
- Intracellular injection of t he catalytic subunit of cyclic AMP-dependent protein kinase simulates facilitation of transmitter release underlying behavioral sensitization in Aplysia.Proceedings of the National Academy of Sciences, 1980
- Microinjection of catalytic subunit of cyclic AMP-dependent protein kinase enhances calcium action potentials of bag cell neurons in cell cultureProceedings of the National Academy of Sciences, 1980
- Effects and Mechanisms of Polypeptide Neurotoxins that Act PresynapticallyAnnual Review of Pharmacology and Toxicology, 1980
- Mechanism of cholera toxin action: Covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase systemProceedings of the National Academy of Sciences, 1978
- Influence of cholera toxin on the regulation of adenylate cyclase by GTPBiochemical and Biophysical Research Communications, 1977
- Poly (ADP-Ribose) and ADP-Ribosylation of ProteinsAnnual Review of Biochemistry, 1977
- Isolation of pure cholinergic nerve endings from the electric organ of Torpedo marmorataBiochemical Journal, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Recent Studies on the Comparative Biochemistry of the Cholinergic NeuronPublished by Cold Spring Harbor Laboratory ,1976