Carbon and Nitrogen Utilization by Rhizopus oligosporus

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Abstract
The utilization of various C and N compounds by Rhizopus oligosporus Saito NRRL 2710 was investigated. The principal components of the soluble carbohydrates of soybeans, i.e., stachyose, raffinose, and sucrose, were not utilized as sole sources of C. Common sugars such as glucose, fructose, galactose, and maltose supported excellent growth as did xylose. Various vegetable oils could be substituted for sugars as sources of C with excellent growth. Ammonium salts and such amino acids as proline, glycine, aspartic acid, and leucine were excellent sources of N. Other amino acids were less suitable, and tryptophan supported no growth at all. Sodium nitrate was not utilized as the sole source of N. Experiments with citrate-buffered media demonstrated that R. oligosporus grows well in a pH as low as 2.6 to 3.0.

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