Characteristics and Calibration of Conductivity Detection in Capillary Electrophoresis

Abstract
The characteristics of end-column conductivity detection in capillary electrophoresis are investigated using solutes and background electrolytes that possess a range of mobilities. Indirect detection is most sensitive for low- to medium-mobility solutes. Detection limits are 1 × 10-6M for hexane sulfonate, and the response is linear for over two orders of magnitude. A generalized response expression is developed for conductivity detection based on the similarities in conductivity and electrophoretic mobility.

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