Circular Epstein-Barr virus genomes of reduced size in a human lymphoid cell line of infectious mononucleosis origin

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Abstract
Circular Epstein-Barr virus (EBV) DNA molecules were purified and characterized from a human lymphoid cell line derived from a case of heterophile antibody-positive, blood transfusion-induced infectious mononucleosis, 883L. The circular EBV DNA in 3 cell lines obtained by transformation of human umbilical cord blood leukocytes with a strain of EBV originally derived from 883L was also studied. As estimated from sedimentation velocity data and EM, the circular EBV DNA molecules are 10-15% smaller than the circular EBV DNA previously found intracellularly in several other types of EBV-transformed cells or the linear EBV DNA present extracellularly in virus particles. The EBV-transformed cord blood cell lines studied here differed from other EBV-transformed [human Burkitt''s lymphoma Raji and P3HR-1 cells] cells in that integrated virus DNA sequences could not be detected.