X-RAY-SENSITIVITY OF 53 HUMAN-DIPLOID FIBROBLAST CELL STRAINS FROM PATIENTS WITH CHARACTERIZED GENETIC-DISORDERS

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (3) , 920-925
Abstract
The in vitro response of 53 human diploid fibroblast strains to X-irradiation was studied using a clonogenic survival assay. The strains, derived from patients with a variety of characterized clinical conditions, most with a genetic component, ranged in D0 [mean lethal dose] (a measure of the slope of the survival curve) from 43-168 rads. The mean D0 of 6 strains from normal individuals was 140-152 rads, with an overall range, based on the extremes of their SE, of 128-164 rads. Of the strains studied 3/4 fell within this range. Strains identified as sensitive came from patients with ataxia telangiectasia, progeria, the 2 genetic forms of retinoblastoma and partial trisomy of chromosome 13. No marked radiosensitivity was found among strains derived from patients with a number of other conditions associated with a predisposition to malignancy. [The use of genetic diseases as models for DNA involvement in carcinogenesis is discussed.].