Mycoplasma cloacale, a New Species Isolated from a Turkey

Abstract
A mycoplasma designated strain 383T(T = type strain) was isolated from the cloaca of a turkey poult. After examination of its morphological, physical, and cultural properties this organism was assigned to the class Mollicutes, order Mycoplasmatales. The base composition of its deoxyribonucleic acid was 26.0 mol% guanine plus cytosine. Strain 383Twas dependent on sterol for growth and was inhibited by digitonin. Since it gave no evidence of helical forms or of urea hydrolysis, it was assigned to the family Mycoplasmataceae, genus Mycoplasma. This organism hydrolyzed arginine and reduced triphenyltetrazolium chloride under anaerobic conditions, but other biochemical tests were negative. Strain 383Tcould not be identified as any of 83 currently accepted Mycoplasma. species or serovars by growth inhibition, immunofluorescence, immunodiffusion, or metabolism inhibition tests. Thus, this mycoplasma appears to be a new species, for which we propose the name Mycoplasma cloacale; the type strain is strain 383 (= NCTC 10199 = ATCC 35276).

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