A simple cannulation technique for determination of accurate blood ethanol clearance rates in rats.

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Abstract
To study the ethanol metabolism of rats in vivo, posthepatic caval blood was sampled by cannulation via the iliolumbar vein, the cannula being exteriorized at the back of the neck, allowing free movement of the animal. The technique minimized stress and permitted repeated sampling of the posthepatic caval blood for up to 60 days. Blood ethanol clearance curves obtained using the cannula gave realistic values for total body water content, indicating that ethanol removal rates calculated from the curves accurately reflected the rate of ethanol metabolism in intact rats.