1. The unequivocal chemical composition of sporopollenin is unknown. Ir was often hypothesized that sporopollenin represents a derivative of carotenoids and/or carotenoid esters. Proceeding from this working hypothesis the influence of inhibitors of carotenoid biosynthesis (DPA, nicotine, Sandoz) on the sporopollenin accumulation was studied in order to test whether an intact carotenoid metabolism is involved. 2. Drastic changes in carotenoid spectra as well as in carotenoid patterns in pollen extracts were used as a “marker” for the uptake and transport of the inhibitors into the anther loculus, the site of sporopollenin biosynthesis. Of the three inhibitors of carotenoid metabolism, DPA, nicotine and Sandoz, only Sandoz appeared to influence carotenoid metabolism in the anthers. 3. After application of Sandoz the sporopollenin accumulation in anthers and pollen was only slightly affected. The amount of sporopollenin after treatment is similar to, or possibly even marginally higher than the control, if dry weight is chosen as reference. If the amount is expressed with respect to one pollen grain, the content is very slightly decreased in the case of pollen from treated plants. Therefore, it is concluded that severe interference in carotenoid biosynthesis does not result in a subsequent drastic inhibition of sporopollenin biosynthesis. 4. A significant increase of PAL activity under the influence of Sandoz indicates that effects upon phenylpropanoid metabolism are possible.