Ceramide inhibits CCN2 expression in fibroblasts
- 1 June 2008
- journal article
- research article
- Published by Wiley in Journal of Cell Communication and Signaling
- Vol. 2 (1-2) , 19-23
- https://doi.org/10.1007/s12079-008-0024-4
Abstract
Connective tissue growth factor (CTGF, CCN2) is induced in response to TGFβ in fibroblasts. In this report, we show that C2 ceramide reduced the ability of TGFβ to induce CCN2 protein, mRNA and promoter activity in fibroblasts. C2 ceramide reduced the ability of TGFβ to induce the generic Smad responsive promoter/reporter construct SBE-luciferase. These results suggest that C2 ceramide reduces the action of TGFβ in fibroblasts via Smad antagonism.Keywords
This publication has 26 references indexed in Scilit:
- Cytotoxicity of TNFα is regulated by integrin-mediated matrix signalingThe EMBO Journal, 2007
- CCN2 Is Necessary for Adhesive Responses to Transforming Growth Factor-β1 in Embryonic FibroblastsJournal of Biological Chemistry, 2006
- CCN2 (Connective Tissue Growth Factor) Promotes Fibroblast Adhesion to FibronectinMolecular Biology of the Cell, 2004
- Treatment with α-Galactosylceramide Attenuates the Development of Bleomycin-Induced Pulmonary FibrosisThe Journal of Immunology, 2004
- Connective Tissue Growth Factor Gene RegulationJournal of Biological Chemistry, 2003
- CTGF expression in mesangial cells: Involvement of SMADs, MAP kinase, and PKCKidney International, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- The control of ccn2 (ctgf) gene expression in normal and scleroderma fibroblastsMolecular Pathology, 2001
- Distinct adapter proteins mediate acid versus neutral sphingomyelinase activation through the p55 receptor for tumor necrosis factorJournal of Leukocyte Biology, 1998
- Regulation of connective tissue growth factor gene expression in human skin fibroblasts and during wound repair.Molecular Biology of the Cell, 1993