The sensitization potential of D & C Yellow No. 11 in guinea pigs

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Abstract
The certified drug and cosmetic [D+C] dye substance known as D and C Yellow No. 11 [2-(2-quinolyl)-1,3 indandione] was tested for its dermal sensitization potential in guinea pigs. An induction regimen consisting of a single footpad injection of complete Freund''s adjuvant diluted with antigen, and an intradermal injection of antigen, produced delayed-type hypersensitivity reactions. A high sensitization frequency was observed with 50 .mu.g D and C Yellow No. 11 and the positive control 2,4-dinitrochlorobenzene (DNCB). The reaction severities of both DNCB and 50 .mu.g D and C Yellow No. 11 were comparable. A dose-response profile was obtained with D and C Yellow No. 11 employing several concentrations (50, 250 and 500 .mu.g/ml). Histologic examination of selected skin sites demonstrated a cellular inflammatory response which was consistent with delayed-type hypersensitivity.