Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli

Abstract
The replication of an F′ plasmid in a dnaC mutant, thermolabile for initiation of chromosomal replication, has been re-examined using a novel DNA-DNA annealing assay. Plasmid replication ceases rapidly at non-permissive conditions, consistent with a direct role for the dnaC product in the replication of F.