Nuclear Sex Identification in Human Tissues: A Histologic Study Using Quinacrine Fluorescence

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Abstract
Frozen sections of tissues of male and female origin were stained with quinacrine dihydrochloride and examined by fluorescence microscopy. A sex difference of nuclei was readily observed. In 71% of interphase nuclei from men a brightly fluorescent spot could be identified, whereas only 9% of nuclei from women had a similar structure. The spermatocytes were exceptional among cells from men because the fluorescent spot was found in only 39% of the nuclei. The fluorescent spot in interphase nuclei indicates the presence of the Y chromosome. This new staining technic will be helpful for identification of nuclear sex when the conventional sex chromatin test is equivocal.

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