Microbial Production of 3aα-H-4α-(3′-Propionic acid)-5α-hydroxy-7aβ-methylhexahydro-1-indanone-δ-lactone from Soybean Sterol

Abstract
Fermentative production of 3aα-H-4α-(3′-propionic acid)-5α-hydroxy-7aβ-methylhexa-hydro-1-indanone-δ-lactone (HIL) from soybean sterol was studied in order to use it as an intermediate for chemical synthesis of 19-norsteroids. A mutant of Nocardia corallina converted 20 g/liter of soybean sterol into 2.8 g/liter of HIL with a 25% yield on a molar basis. The dominant factors improving the productivity were the use of an amino acid mixture as a nitrogen source and the preparation of the sterol suspension by sonication or with surface-active agents.

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