The metabolism of deoxyguanosine in mitochondria. Characterization of the uptake process
- 1 January 1987
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 77 (1) , 71-77
- https://doi.org/10.1007/bf00230152
Abstract
The uptake of deoxyguanosine by rat liver mitochondria was characterized. The process required an intact mitochondrial membrane and exhibited a dependence on added phosphate. Deoxyguanosine uptake was minimally influenced by Mg2+ or Mn2+, but Ca2+ at concentrations above 0.5 mM were detrimental. Of the deoxynucleosides tested, only deoxyinosine inhibited the uptake of deoxyguanosine. The ribonucleoside guanosine was not observed to compete with its deoxynucleoside analog. Known inhibitors of nucleoside transport, cytochalasin B and NBMPR, did not block deoxyguanosine uptake, but the sulfhydryl reagents NEM and pCMB were both inhibitory. The uptake of deoxyguanosine was shown to be a saturable process and an apparent Km of 0.64 σM was calculated from a Hanes plot.This publication has 15 references indexed in Scilit:
- Species differences in nucleoside transport. A study of uridine transport and nitrobenzylthioinosine binding by mammalian erythrocytesBiochemical Journal, 1982
- Regulation of Deoxynucleoside Kinase Activities in Rat Liver MitochondriaEnzyme, 1982
- Nucleoside transport in human and sheep erythrocytes. Evidence that nitrobenzylthioinosine binds specifically to functional nucleoside-transport sitesBiochemical Journal, 1980
- Deoxyguanosine kinase. Distinct molecular forms in mitochondria and cytosolJournal of Biological Chemistry, 1979
- Mechanism of action of agents which uncouple oxidative phosphorylation: Direct correlation between protoncarrying and respiratory-releasing properties using rat liver mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1975
- Mediated transport of nucleosides in human erythrocytes. Specific binding of the inhibitor nitrobenzylthioinosine to nucleoside transport sites in the erythrocyte membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- Deoxyribonucleic acid biosynthesis in mitochondria. Studies on the incorporation of labeled precursors into mitochondrial deoxyribonucleic acid.1973
- Replication of Mitochondrial DNA in Mouse L Cells and Their Thymidine Kinase - Derivatives: Displacement Replication on a Covalently-Closed Circular TemplateProceedings of the National Academy of Sciences, 1972
- Persistence of Thymidine Kinase Activity in Mitochondria of a Thymidine Kinase-Deficient Derivative of Mouse L CellsProceedings of the National Academy of Sciences, 1972
- Cytochalasin B: Inhibition of Glucose and Glucosamine TransportProceedings of the National Academy of Sciences, 1972