A First-Generation Whole Genome–Radiation Hybrid Map Spanning the Mouse Genome

Abstract
We have assembled a first-generation anchor map of the mouse genome using a panel of 94 whole-genome–radiation hybrids (WG–RHs) and 271 sequence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb apart on average. This mouse WG–RH panel, known as T31, has an average retention frequency of 27.6% and an estimated potential resolution of 145 kb, making it a powerful resource for efficient large-scale expressed sequence tag mapping.[All of the mapping data for the maps presented here have been deposited at the Research Genetics, Inc., web site and can be freely accessed and downloaded athttp://www.resgen.com/.]