Abstract
Marker frequency analysis by mutation induction of several strains of Staphylococcus aureus has yielded a genomic map supporting the map derived by another technique. Gene orders of exponential cells obtained by using reference cells with completed chromosomes effected by chloramphenicol or by phenethanol treatment were identical, suggesting that these two agents halted chromosomal replication at a common terminus. In several strains, the pantothenate or acriflavine resistance locus changed its apparent chromosomal position during exponential growth. The data indicate that more than one chromosomal growing point occurred during logarithmic growth in non-synthetic medium.

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