Fabrication of Novel Electrochemical Reduction Systems Using Alcohol Dehydrogenase as a Bifunctional Electrocatalyst

Abstract
Electrochemical reduction of NADP+ to NADPH and of NAD+ to NADH with current efficiencies of more than 97% has been achieved at alcohol dehydrogenase (ALDH) in the presence of acetophenone as an electron mediator. Addition of acetone or acetaldehyde as a substrate to the above electrolytic system allowed reduction of the substrate to the corresponding alcohol at ALDH accompanied by oxidation of the resulting NAD(P)H.