An Endogenous Ovarian Growth Factor Which Stimulates BALB/3T3 and Granulosa Cell Proliferation
- 1 December 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 29 (5) , 1135-1141
- https://doi.org/10.1095/biolreprod29.5.1135
Abstract
Extracts of whole porcine ovary stimulate mouse BALB/3T3 [3H]DNA synthesis. Biochemical characterization indicates the growth factor is associated with a soluble cationic protein that is labile to heat, stable to dithiothreitol treatment and elutes from Sephadex G-100 between chymotrypsin (25,000 MW) and cytochrome c (12,700 MW). Extracts of thecal tissue have growth-promoting activity in both granulosa and 3T3 cells. Conditioned medium from thecal but not granulosa cell cultures stimulated [3]DNA synthesis in quiescent 3T3 and granulosa cells. The presence of an endogenous protein ovarian growth factor found in the theca that stimulates granulosa cell proliferation in vitro is implicated.This publication has 10 references indexed in Scilit:
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