Quantitative Method for Estimating Fecal Amino Acids
Open Access
- 1 September 1975
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 21 (10) , 1437-1440
- https://doi.org/10.1093/clinchem/21.10.1437
Abstract
We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 µmol of α-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automated ion-exchange column chromatography of the dialysate. Some normal values are provided.Keywords
This publication has 0 references indexed in Scilit: