The Purification of Red Cells for Transfusion by Freeze‐Preservation and Washing V. Red Cell Recovery and Residual Leukocytes after Freeze‐Preservation with High Concentrations of Glycerol and Washing in Various Systems
- 2 January 1977
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 17 (1) , 1-7
- https://doi.org/10.1046/j.1537-2995.1977.17177128877.x
Abstract
Red blood cells freeze‐preserved with 40% W/V glycerol in an ionic or low ionic medium at −80C were washed in one of four commercially available systems: the Haemonetics Blood Processor 15 using a continuous‐flow centrifugation procedure, the Fenwal Elutramatic using a continuous‐flow centrifugation procedure, the IBM Blood Processor using an automated serial centrifugation procedure, and the Huggins Cytoglomerator using a dilution/agglomeration procedure. Determinations of red blood cell recovery and leukocyte and platelet removal were made for each of these groups. The dilution/agglomeration procedure produced lower red blood cell recovery values and poorer leukocyte and platelet removal than did any of the three wash systems using sodium chloride solutions. The values obtained with the systems using sodium chloride solutions were slightly but significantly different.This publication has 21 references indexed in Scilit:
- The Purification of Red Cells for Transfusion by Freeze‐Preservation and Washing. IV. The Use of Micropore Filtration To Reduce the Residual HL‐A Antigenicity of Previously Frozen, Washed Red CellsTransfusion, 1975
- The Purification of Red Cells for Transfusion by Freeze‐Preservation and WashingTransfusion, 1974
- The Purification of Red Cells for Transfusion by Freeze Preservation and WashingTransfusion, 1974
- The Purification of Red Cells for Transfusion by Freeze Preservation and WashingTransfusion, 1974
- BLOOD TRANSFUSIONS AND UNRESPONSIVENESS TO HL-ATransplantation, 1973
- Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor BloodTransfusion, 1973
- Red Cell Recovery and Leukocyte Depletion Following Washing of Frozen‐Thawed Red CellsTransfusion, 1973
- Simple Methods for Production of HL‐A Antigen Poor Red Blood CellsTransfusion, 1973
- Leukocyte Contamination of Red Cells in Leukocyte‐Poor and Frozen‐Deglycerolized UnitsTransfusion, 1973
- Transfusion of Buffy Coat‐Poor Red Cell Suspensions Prepared by Dextran Sedimentation: Description of Newly Designed Equipment and Evaluation of Its UseTransfusion, 1962