Detection of HBsAg in a clone derived from the PLC/PRF/5 human hepatoma cell line
- 1 September 1981
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 68 (3-4) , 157-163
- https://doi.org/10.1007/bf01314568
Abstract
A total of 28 clones were established from the PLC/PRF/5 hepatoma cell line by a plating procedure. All clones were found to secrete HBsAg into the supernatant culture fluids. Of these, one clone (No. 23) free of detectable mycolasma contamination and showing smooth epithelial morphology was selected for further study. Maximum accumulation of HBsAg occurred 9 days after sub-culture and intracellular antigen was detected by indirect immunofluorescence both in the cytoplasm and at the plasma membrane. Granules and perinuclear staining reactions were also observed in clone 23 cells and these findings are compared to the previously published properties of the parental PLC/PRF/5 cell line.This publication has 13 references indexed in Scilit:
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