Simple Assay for Accurate Determination of [ 35 S]sulfate Reduction Activity
- 1 June 1983
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 45 (6) , 1956-1959
- https://doi.org/10.1128/aem.45.6.1956-1959.1983
Abstract
A method is described for the assay of [ 35 S]sulfate reduction in which filter paper wicks are used to trap [ 35 S]sulfide. The simplicity of the technique enables large numbers of samples to be conveniently processed. Enhanced sensitivity is achieved since all acid-volatile [ 35 S]sulfides produced during the incubation period are counted. Recovery of radioactivity from added Na 2 35 S is excellent (mean, 100.1%; standard deviation, 1.81; n = 9) and is unaffected by sulfide concentrations of up to 400 μg per sample. Field trial results with anoxic sediment samples are presented.Keywords
This publication has 2 references indexed in Scilit:
- Measurement and Significance of Specific Activity in the Heterotrophic Bacteria of Natural WatersApplied and Environmental Microbiology, 1978
- Sulfate uptake as a measure of planktonic microbial production in freshwater ecosystemsCanadian Journal of Microbiology, 1974