QUANTITATION OF BOVINE IMMUNOGLOBULINS - COMPARISON OF SINGLE RADIAL IMMUNODIFFUSION, ZINC-SULFATE TURBIDITY, SERUM ELECTROPHORESIS, AND REFRACTOMETER METHODS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (5) , 693-698
Abstract
Laboratory methods designed to quantitate serum immunoglobulin [Ig] were evaluated: single radial immunodiffusion [RID], ZnSO4 turbidity and serum electrophoresis. Estimation of Ig concentration in neonatal calves, using total protein measurements, was also evaluated. Single RID proved useful for quantitation when class or subclass information was needed. ZnSO4 turbidity measurements gave accurate results for total Ig except when hemolysis was present in the sample. A correction factor for Hb that should minimize errors resulting from hemolysis was presented. Serum electrophoresis was an accurate quantitation method. Large errors were encountered in attempting to estimate Ig on the basis of total protein.

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