TWELVE-HOUR AND TWENTY FOUR-HOUR PRESERVATION OF SMALL BOWEL ALLOGRAFTS BY SIMPLE HYPOTHERMIA

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Abstract
Canine small bowel was harvested and stored by simple hypothermic technique. After 12-and 24-hr storage, respectively, the small bowel graft was allotransplanted into recipients. All animals receiving 12-hr stored grafts (n=9) survived beyond 5 days. In the 24-hr storage group, 67% of the animals (n=9) survived beyond 5 days. Successful storage for such extended periods by simple hypothermia has not been achieved previously. Donor pretreatment with antibiotics as well as extensive intra-luminal irrigation of the harvested small bowel are considered to be important technical features in this successful preservation.

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