Effects of Neuropeptide Y on the in vitro Release of Gonadotropin-Releasing Hormone, Luteinizing Hormone, and Beta-Endorphin and Pituitary Responsiveness to Gonadotropin-Releasing Hormone in Female Macaques

Publisher Website
Google Scholar
Abstract
The objectives of these studies were to examine the release of gonadotropin-releasing hormone (GnRH) and β-endorphin-like activity (β-EP) from macaque hypothalami, and the release of luteinizing hormone (LH) and GnRH-induced LH from macaque anterior pituitaries in response to neuropeptide Y (NPY) treatment. Anterior hypothalamic (AH) and mediobasal hypothalamic (MBH) blocks of tissues and the adenohypophysis were bisected along the midline into two equal-sized fragments. Fragments were superfused with medium for 3 h, followed by 3 h of either NPY (80 nM) or medium alone. In a separate experiment, adenohypophyseal (AP) fragments were superfused in accordance with the same protocol (3 h medium – 3 h NPY or medium) except that exogenous GnRH (352 nM) was added for 30 min at the beginning of hour 3 and again at the beginning of hour 6. Immunoactive GnRH, β-EP, and LH levels were measured in superfusate samples (400 µl) collected at 10-min intervals. GnRH levels rose within 20–30 min of initiation of NPY treatment, and elevated GnRH release was sustained for the duration of NPY exposure of both AH and MBH fragments from ovarian intact (INT) rhesus (Macaca mulatta; n = 8; p Macaca fascicularis; n = 4; p 0.05; n = 7). NPY treatment had no effect on β-EP release from AH, MBH, and AP tissues of either INT or OVX rhesus macaques. These findings suggest that (1) the stimulatory action of NPY on GnRH release in macaque hypothalami and LH release in macaque anterior pituitaries requires functioning ovaries; (2) NPY does not enhance the sensitivity of macaque gonadotropes to GnRH stimulation, and (3) the mechanism of stimulatory NPY action may not involve the neuronal release of β-EP.

This publication has 0 references indexed in Scilit: