Chromosome sorting and DNA sequence localization
- 1 November 1982
- Vol. 3 (3) , 145-154
- https://doi.org/10.1002/cyto.990030302
Abstract
This article focuses on current techniques and possible future developments in chromosome isolation and sorting, and DNA analysis of sorted chromosomes. The strategy of subchromosomal gene mapping by chromosome sorting is outlined and a list of cell lines containing translocated chromosomes is provided which may be used to map genes to a single chromosome with a standard fluorescence activated cell sorter. The usefulness of this and other gene mapping methods for localizing unique DNA sequences and characterizing recombinant DNA libraries constructed from sorted chromosomal DNA is also discussed.Keywords
This publication has 55 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Design and operation of a dual laser chromosome sorterCytometry, 1982
- Isolation of mouse X‐chromosome specific DNA from an X‐enriched lambda phage library derived from flow sorted chromosomesCytometry, 1982
- Human chromosome isolation from short-term lymphocyte culture for flow cytometryNature, 1981
- Rapid isolation of metaphase chromosomes containing high molecular weight DNA.The Journal of cell biology, 1979
- Isolation of specific human metaphase chromosomesBiochemical and Biophysical Research Communications, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Purification of the chromosomes of the Indian muntjac by flow sorting.Journal of Histochemistry & Cytochemistry, 1976
- PARASEXUAL APPROACHES TO THE GENETICS OF MANAnnual Review of Genetics, 1975
- New method for mapping genes in human chromosomesNature, 1975