Cryoprecipitated antihemophilic factor production from blood collected in quad packs or from blood with delayed processing. The importance of plasma thawing method

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Abstract
Two alternate approaches to increasing the supply of plasma suitable for the production of cryoprecipitated AHF (cryo) were evaluated. In the first, cryo was prepared from blood collected in quadruple packs from which red cells and platelets also were made. This procedure resulted in a mean reduction of starting plasma volume of about 25 percent with a concomitant decrease in factor VIII coagulant activity (FVIII:C) of cryo when compared to approximately 2000 cryos made in triple packs assayed in our laboratory during the past year. However, American Red Cross (ARC) regions using a higher yield waterbath method of thawing plasma successfully produced quadruple pack cryos with a mean potency of 109 international units per container and about 90 percent of them met federal potency requirements. This was not the case for ARC regions using the lower yield method of thawing plasma in a refrigerator. The second approach involved cryo production from plasma frozen following 15 hours of cold storage of either the separated plasma or whole blood. Cold storage of plasma resulted in small but insignificant decreases in FVIII:C potency and yield in the cryo. However, there was a 20 percent decrease in FVIII:C yield in cryo produced from frozen plasma following 15 hours of cold storage of whole blood. Again, the decrease in FVIII:C yield resulting from interim blood storage was compensated for by the use of the higher yield waterbath method.